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To induce superovulation, mice were injected intraperitoneally with 10 IU of pregnant mare serum gonadotropin (PMSG), followed 48 hours later by 10 IU of human chorionic gonadotropin (hCG)

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doi:10.1186/s12902-022-01114-3 [18] Frhlich E, Wahl R

Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template